| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Dunnellon |
B | Charles | Henry | Vickers | Simpson | NT |
| 3 | Zephyrhills |
B | Redfield | Campbell | Williams-Bennie | Sands | NT |
| 4 | Mount Dora Christian |
B | Banks | Betances | Weilbacher | Napoli | NT |
| 5 | Nature Coast |
B | Mendez | Mangozho | Warford | Jackson | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Hernando |
A | Sansone | Brown | Capel III | Dotson | DQ |
| 2 | Weeki Wachee |
A | Wilson | Worman | Somers | Garcia | 45.57 |
| 3 | Nature Coast |
A | Picariello | Harvin | Montgomery | Mckenzie | 43.75 |
| 4 | South Sumter |
A | Overton | Gibson | Young | Johnson | 42.85 |
| 5 | Zephyrhills |
A | Woodard | Norton | Miller | Jackson | 43.18 |
| 6 | Mount Dora Christian |
A | Abbott | Roach | Roach | Taksey | 44.10 |
| 7 | Dunnellon |
A | Sanchez | Jackson | Lanier | Newby | 51.52 |