| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 |  Dunnellon | 
		B | Charles | Henry | Vickers | Simpson | NT | 
| 3 |  Zephyrhills | 
		B | Redfield | Campbell | Williams-Bennie | Sands | NT | 
| 4 |  Mount Dora Christian | 
		B | Banks | Betances | Weilbacher | Napoli | NT | 
| 5 |  Nature Coast | 
		B | Mendez | Mangozho | Warford | Jackson | NT | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 |  Hernando | 
		A | Sansone | Brown | Capel III | Dotson | DQ | 
| 2 |  Weeki Wachee | 
		A | Wilson | Worman | Somers | Garcia | 45.57 | 
| 3 |  Nature Coast | 
		A | Picariello | Harvin | Montgomery | Mckenzie | 43.75 | 
| 4 |  South Sumter | 
		A | Overton | Gibson | Young | Johnson | 42.85 | 
| 5 |  Zephyrhills | 
		A | Woodard | Norton | Miller | Jackson | 43.18 | 
| 6 |  Mount Dora Christian | 
		A | Abbott | Roach | Roach | Taksey | 44.10 | 
| 7 |  Dunnellon | 
		A | Sanchez | Jackson | Lanier | Newby | 51.52 |