| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 1 | LAMPRECHT, Gabiella | SO | Parrish | NT | 
| 2 | SOUNDARA, Nirvana | JR | Palmetto | NT | 
| 3 | KENNEDY, Ja'Nya | SO | Booker | NT | 
| 4 | BUNDRAGE, Ronasia | SO | Braden River | NT | 
| 5 | HAYES, Lily | SO | Parrish | NT | 
| Lane | Athlete | Yr. | Team | Seed | 
|---|---|---|---|---|
| 2 | ALFLEN, Kassidy | SO | Braden River | 1:04.08 | 
| 3 | SANTIAGO, Sofia | JR | Booker | 55.86 | 
| 4 | WATTS, Kalea | SO | Palmetto | 53.01 | 
| 5 | HOPKINS, Eleanor | JR | Palmetto | 55.00 | 
| 6 | DUSSAULT, Emma | SO | Braden River | 1:02.80 |