| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Manatee |
A | SMITH | Padilla | PRINCE | PATTERSON | NT |
| 3 | Southeast |
A | Derusseau | Davila | Lewis | Byrd | 3:40.47 |
| 4 | Palmetto |
A | Roberson | Ashton | Ashton | Starling | 3:30.00 |
| 5 | St. Stephens Episcopal |
A | Riedi | Christian | Riedi | Carr | 3:36.35 |
| 6 | Babcock | A | Blocker | Rubiano | Lundy | Miret | 4:09.33 |