| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Lake Minneola | A | Rhymes | Celestin | Ramos | Connor | NT |
| 2 | Mount Dora | A | Scott | Fourari | Zwart | Ficket | NT |
| 3 | South Lake | A | Allen | Trewin | McKinnond | 44.97 | |
| 4 | South Sumter | A | Overton | Hill | Young | Dorsey | 45.11 |
| 5 | Tavares | A | Lowman | Brooks | Smith | Benoit | 47.04 |
| 6 | Umatilla | A | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | East Ridge | A | Facey | Farrell | Patterson | McCormick | 44.35 |
| 2 | Mount Dora Christian | A | Andrews | Schick | Dale | Bennett | 43.88 |
| 3 | Wildwood | A | Best | Smith | Brown III | Colebrooks | 42.95 |
| 4 | Villages | A | Sarakinis | Isaac | Rojas | Globig | 43.50 |
| 5 | Leesburg | A | Chapman | Hammonds | Filamar | Filamar | 44.33 |
| 6 | Eustis | A | Patterson | Wilder | Vega | Sayles | 44.39 |