| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 |  Weeki Wachee | 
		A | Hessler | Anderson | Williams | Randazzo | NT | 
| 2 |  Dunnellon | 
		A | Hatcher | De La Rosa | Fenton | Alvord | 4:49.13 | 
| 3 |  South Sumter | 
		A | Cruz | Davis | Rodgers | Guevarra | 4:34.12 | 
| 4 |  Armwood | 
		A | Thornton | Ankers | Allen | Archie | 4:36.92 | 
| 5 |  Nature Coast | 
		A | Dardignac | Anderson | Sanders | Smith | 4:51.53 | 
| 6 |  Crystal River | 
		A | Broadbent | Cordero-Santana | Johnson | Santiago | 5:04.78 |