| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Hernando |
A | Gill | Branon | Nixon | Delmont | 55.57 |
| 2 | Central (Brooksville) |
A | Richardson | Rodriguez Baez | Jordan | Burton | NT |
| 3 | Weeki Wachee |
A | Anderson | Clark | Cox | Randazzo | 55.92 |
| 4 | Hudson |
A | Heintzelman | Pipe | Jenkins | Gober | DQ |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Dunnellon |
A | Bolton | Burns | Hall | Matthews | 53.98 |
| 2 | Nature Coast |
A | Perez Cruz | Schotborgh | Lawson | Norton | 53.33 |
| 3 | Armwood |
A | Fulton | Tolbert | Hamilton | Sterling | 49.07 |
| 4 | South Sumter |
A | Cruz | Guevarra | Solomon | Caldwell | 51.31 |
| 5 | Pasco |
A | Carter | James | Richardson | White | 53.49 |