| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Dunnellon |
A | Elijah | Martin | Rollerson | Henry | 48.09 |
| 2 | Central (Brooksville) |
A | Redmile | Coar | Graham | Wade | DQ |
| 3 | Weeki Wachee |
A | Wilson | Wildgoose | Ferrugia-Hughes | Hodge-Mauldin | 46.10 |
| 4 | Pasco |
A | Norris | Freeman | Orane | Stinyard | 46.10 |
| 5 | Bishop McLaughlin |
A | Tarrio | Jorgensen | Ledger | Chalk | 48.07 |
| 6 | Crystal River |
A | Hamilton | Richardson | Verschraegen | Robinson | 49.18 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Hernando |
A | Dotson | Scrivens-Mobley | Foulks | Capel III | DNF |
| 2 | Nature Coast |
A | Harvin | Thomas | Montgomery | Mckenzie | DQ |
| 3 | Armwood |
A | Anderson | Ford | Pringle | Smith | 42.03 |
| 4 | South Sumter |
A | Overton | McDonald | Young | Johnson | NT |
| 5 | Hudson |
A | Ramcharitar | Keeling | Jackson | Conley | 44.81 |
| 6 | Citrus |
A | Blackburn | Dunois | Martone | Schuchard | NT |