| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 |  Dunnellon | 
		A | Elijah | Martin | Rollerson | Henry | 48.09 | 
| 2 |  Central (Brooksville) | 
		A | Redmile | Coar | Graham | Wade | DQ | 
| 3 |  Weeki Wachee | 
		A | Wilson | Wildgoose | Ferrugia-Hughes | Hodge-Mauldin | 46.10 | 
| 4 |  Pasco | 
		A | Norris | Freeman | Orane | Stinyard | 46.10 | 
| 5 |  Bishop McLaughlin | 
		A | Tarrio | Jorgensen | Ledger | Chalk | 48.07 | 
| 6 |  Crystal River | 
		A | Hamilton | Richardson | Verschraegen | Robinson | 49.18 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 1 |  Hernando | 
		A | Dotson | Scrivens-Mobley | Foulks | Capel III | DNF | 
| 2 |  Nature Coast | 
		A | Harvin | Thomas | Montgomery | Mckenzie | DQ | 
| 3 |  Armwood | 
		A | Anderson | Ford | Pringle | Smith | 42.03 | 
| 4 |  South Sumter | 
		A | Overton | McDonald | Young | Johnson | NT | 
| 5 |  Hudson | 
		A | Ramcharitar | Keeling | Jackson | Conley | 44.81 | 
| 6 |  Citrus | 
		A | Blackburn | Dunois | Martone | Schuchard | NT |