| Lane | # | Athlete | Team |
|---|---|---|---|
| 1 | 406 | WELLS, Caroline | Winter Springs |
| 2 | 857 | WYANT, Ava | Master's Academy (Oviedo) |
| 3 | 267 | DEJESUS, Grace | Circle Christian |
| 4 | 603 | REBURN, Claire | Foundation Academy |
| 5 | 1004 | HEALD, Maddie | Olympia |
| 6 | 344 | WARREN, Hanna | East Ridge |
| 7 | 227 | MOURY, Leah | Circle Christian |
| 8 | 41 | LONGSTAFF, Abigail | Orangewood Christian |
| 9 | 313 | MALDONADO, Lina | East Ridge |
| 10 | 180 | MANIDIS, Adriana | Lake Brantley |
| 11 | 303 | MCCORMACK, Madison | East Ridge |
| 12 | 58 | MCCULLY, Emma | Orangewood Christian |
| 13 | 804 | VECCHIO, Hildi | Edgewater |
| 14 | 770 | NOTARO, Elizabeth | Edgewater |
| 15 | 669 | RHODES, Maddy | Lyman |
| 16 | 801 | KRENS, Emma | Edgewater |