| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | South Lake |
A | Wagenhauser | Ortiz | Miller | Caurthens-Lowe | 11:34.34 | |
| 2 | Mitchell |
A | Acocella | Crespo | Erickson | Viens | 11:03.53 | West SAC Conference Meet |
| 3 | Wiregrass Ranch |
A | Burkett | Gregory | Bohrer | Pfaltzgraff | 11:11.11 | |
| 4 | Angeline Academy | A | Booza | Escobar | Sanchez | Harris | 11:53.74 | 2025 Newsome Wolves Invitational |
| 5 | Gulf |
A | Costanzi | Gordon | Voveris | Stevens | 11:12.27 | FHSAA 3A Region 2 |
| 6 | Tarpon Springs |
A | Jarrell | Raeffer | Sarantapoulas | Pope | 10:59.14 | Thomas Jet Jackson Invitational |