| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Challenger K-8 School | A | 1:02.81 | Challenger K8 Host, Fox Chapel, |
| 3 | West Hernando Middle School | A | 56.69 | DS Parrott Host Explorer K8, Wes |
| 4 | Winding Waters K-8 School | A | 58.15 | DS Parrott Host Explorer K8, Wes |
| 5 | Fox Chapel Middle School | A | 1:02.89 | Challenger K8 Host, Fox Chapel, |