| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | King School | A | NT | |
| 4 | Chiefland |
A | 4:13.07 | Chiefland Home Meet 2 |
| 5 | Seven Rivers Christian |
A | 4:16.33 | Citrus Round Up Championship |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Belleview |
A | 3:44.19 | Mount Dora Christian Academy Meet 1 |
| 2 | Eastside |
A | 3:36.61 | 2026 Alachua County Championships |
| 3 | Lakewood |
A | 3:29.13 | Larry Richardson Memorial Invite |
| 4 | Buchholz |
A | 3:26.77 | 2026 Alachua County Championships |
| 5 | Nature Coast |
A | NT | 2026 Kiwanis Invitational |
| 6 | Wildwood |
A | 3:33.69 | Wildwood Friday Night Invitational |
| 7 | Land O'Lakes |
A | 3:42.11 | Coyote Invitational |