| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lecanto |
B | Padgett | Permenter | Rose | Sherman | NT | |
| 2 | Lake Minneola |
D | Bristol | Brown | Edwards | Pope | NT | |
| 3 | Wildwood |
B | Willis | Miller | Wilson | Holmes | 47.50 | |
| 4 | Dunnellon |
D | Mills | Mathis | Hebert | Robinson | NT | |
| 5 | Lake Minneola |
C | Ahmed | Douglas | Smith | Brumfield | NT | |
| 6 | Crystal River |
A | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Dunnellon |
B | Townsend | Dudley | Reed | Abate | NT | |
| 2 | Lecanto |
C | Kaddaf | Hester | Huggins | Rexach | NT | |
| 3 | Lake Minneola |
B | Irving | Denmark | Irvin | Stewart | 45.37 | |
| 4 | Dunnellon |
C | Mims | Cordosa | Sanchez | Council | NT | |
| 5 | Wildwood |
C | Brown | Graham | Pierre | Ruffin | NT | |
| 6 | Seven Rivers Christian |
B | Waller | Whaley | Moore | Summers | 1:05.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Circle Christian |
A | Jones | Atwell | Sill | Keller | NT | Charlie Harris Relay |
| 2 | Lecanto |
A | Boehmer | Morgan | Samms | Griffin | 48.00 | |
| 3 | Dunnellon |
A | Bracey | Woods | Wheeler | Tanner | 44.55 | Wildwood Open |
| 4 | Lake Minneola |
A | Derilus | Lubin | Mounger | Manley | 42.31 | FHSAA 4A Region 1 |
| 5 | Wildwood |
A | Solis | Corbin | Cousizan | Slowley | 43.28 | |
| 6 | Seven Rivers Christian |
A | Mejias | Whaley | Westberry | Gipple | 48.83 | Citrus Round Up Championship |
| 7 | First Academy (Leesburg) |
A | Rutzebeck | Collins | Bone | Lowe | 47.55 | The Villages Home Meet Two |
| 8 | Citrus |
A | Brown | Macias | Cobb | Takett | NT |