| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Note |
|---|---|---|---|---|---|---|---|
| 2 | Dunnellon |
A | Mills | Townsend | Linder | Council | Citrus Round Up Championship |
| 3 | North Marion |
A | Carpenter | Blakey | Griffin | Johnson | South Sumter High School Raider Open |
| 4 | West Port |
A | Rios | Rios | Powell | Lozano | Bob English Invitational 2026 |
| 5 | Wildwood |
A | Corbin | Slowley | Cousizan | Spurrier | Wildwood Friday Night Invitational |
| 6 | Orlando Christian Prep |
A | Verkennes | Kaufman | Mackey | Anderson | Hawk's Early Bird Invitational |
| 7 | First Academy (Leesburg) |
A | Ogden | Collins | Lowe | Rutzebeck | Villages Home Meet One |