| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Hudson |
A | Maher | Mendez | Levasseur | De'Jesus | NT | |
| 3 | Hernando |
A | Delmont | Smith | Dominquez | Bennett | 54.54 | 2026 Hernando Relays |
| 4 | Bishop McLaughlin |
A | Pruitt | Alicea | Desbien | Bianchi | 56.63 | FHSAA 1A Region 3 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Nature Coast |
A | Norton | Schotborgh | Peters | Page | 51.18 | FHSAA 2A District 5 |
| 3 | Wesley Chapel |
A | Denning | Denning | Smith | Joseph | 48.87 | FHSAA 3A District 8 |
| 4 | Central (Brooksville) |
A | Austin | Bailey | Issac | Miodowski | 51.18 | Weekie Wachee Quad meet |
| 5 | Weeki Wachee |
A | Benavente | Anderson | Kernisant | Baker | 53.86 | FHSAA 3A District 8 |