| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 150 | PHILLIPS, Jackson | SO | Robinson |
5:34.41 | Robinson Tri Meet #1 |
| 2 | 175 | PAES, Nicholas | FR | Robinson |
5:36.03 | Robinson Tri Meet #1 |
| 3 | 63 | D'ANGELO, David | JR | Jesuit (Tampa) |
5:37.24 | Jesuit Meet #2 |
| 4 | 54 | HENRY, Tripp | JR | Jesuit (Tampa) |
5:38.72 | Jesuit Meet #4 |
| 5 | 67 | SOBLEMAN, Henry | SO | Jesuit (Tampa) |
5:40.59 | Jesuit Meet #4 |
| 6 | 70 | ARCOS, Finn | FR | Jesuit (Tampa) |
5:45.00 | |
| 7 | 163 | CAPUTO, Pete | SO | Robinson |
5:48.49 | Robinson Tri Meet #1 |
| 8 | 149 | KARALEKAS, Alexander | SO | Robinson |
5:51.46 | Robinson Tri Meet |
| 9 | 88 | GAROFANI, Alex | SO | Jesuit (Tampa) |
6:05.00 | |
| 10 | 44 | FULTZ, Lucas | SO | Jesuit (Tampa) |
6:05.00 | |
| 11 | 39 | DEWEY, Gabriel | SO | Jesuit (Tampa) |
6:05.00 | |
| 12 | 114 | RODRIGUEZ, Mike | SO | Jesuit (Tampa) |
6:05.00 | |
| 13 | 85 | FRANZ, Colton | FR | Jesuit (Tampa) |
6:05.00 | |
| 14 | 73 | BUCK, Ethan | SO | Jesuit (Tampa) |
6:05.00 | |
| 15 | 90 | GENTILE, Alex | SO | Jesuit (Tampa) |
6:05.00 | |
| 16 | 87 | GARCIA, Javier | FR | Jesuit (Tampa) |
6:05.00 | |
| 17 | 254 | DUDAS, Thomas | JR | Tampa Catholic |
6:24.99 | East Lake Early Bird Invitational (Approved Qualifier) |
| 18 | 186 | BARTLEY, Eli | FR | Robinson |
NT | |
| 19 | 171 | BRADDOCK, Hadrian | FR | Robinson |
NT | |
| 20 | 168 | PEREZ, Jaden | SO | Robinson |
NT |