| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Bradenton Christian |
A | Ditzel | Cordell | Delgado | Bailey | 45.92 | FHSAA 1A District 11 |
| 3 | Lemon Bay |
A | Brenner | Sanguinet | Turner | Dobbs | NT | FHSAA 2A Region 3 |
| 4 | Palmetto |
A | Leaks | Scott | Delgado | Perkins | DQ | |
| 5 | Lakewood Ranch |
A | Turski | ZArlenga | Knox | Zarlenga | 43.12 | FHSAA 4A District 11 |
| 6 | Southeast |
A | Gehndyu | Peterson | Greene | Lewis | 44.14 | FHSAA 3A District 9 |
| 7 | Braden River |
A | Sloan | Boyd | Yermak | Getman | 46.39 | 46.39 Manatee County Track and Field Championship |