| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 163 | WOITALEWICZ, RUBY | JR | Lemon Bay |
NT | |
| 3 | 153 | HILGERS, CHLOE | FR | Lemon Bay |
NT | |
| 4 | 382 | CLARK, BRIELLE | FR | Lakewood Ranch |
20.58 | |
| 5 | 392 | JOHNSON, MILANA | SO | Lakewood Ranch |
25.00 | |
| 6 | 518 | BAPTISTE, ZARHIYA | SO | Southeast |
NT | |
| 7 | 203 | STRAHAN, CORA | SO | Lemon Bay |
NT | |
| 8 | 159 | PENSZKI, HANGA | FR | Lemon Bay |
NT |