| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Fivay | A | NT | |||||
| 2 | Gulf | A | Beamon | Scorca | Sannoh | Gonzales | NT | |
| 3 | Mitchell | A | Polen | Koenig | Tropnas | Burnette | NT | |
| 4 | Hudson | A | Shawell | Keeling | Norris | Conley | 43.09 | |
| 5 | River Ridge | A | Atkinson | Patterson | Phillips | Quintana | NT | |
| 6 | Anclote | A | Stroupe | Brown | Waring | Spencer | NT |