| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lakewood Ranch |
A | Baumgartner | Mulligan | Walker | Shear | 10:20.26 | Manatee High Hurricane Invite |
| 2 | Palmetto |
A | Tripp | Zimmerman | Fano | Dunaway | 10:34.20 | Spring Break Invitational 2026 - Approved Qualifier |
| 3 | Bradenton Christian |
A | Schuiteman | Schuiteman | Hughes | Mark | NT | Manatee High Hurricane Invite |