| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Booker |
A | Pierce | Estridge | Haywood | Mobley | NT | 3:46.14 Sarasota Riverview - Ram Invite 2025 |
| 3 | Braden River |
A | Bazo | Clark | Sloan | Kien | 3:37.08 | Larry Richardson Memorial Invite |
| 4 | Palmetto |
A | Xavier | Starling | Ashton | Roberson | NT | 3:34.51 Hurricane Invitational Track and Field Meet |
| 5 | Bayshore |
A | Colston | Davis | Stancil | Wiley | 3:35.12 | |
| 6 | Southeast |
A | Randle | Fields | Walker | Peterson | 3:42.93 | Eddie Starling Memorial Invite |
| 7 | Bradenton Christian |
A | Bailey | McArthur | Sanchez | Mercer | NT | Saint Stephen's Falcon Invite 2026 |