| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 88 | SEVERINO, EMILY | 8 | Bradenton Christian |
NT | |
| 3 | 49 | BYRD, ZENAIYA | FR | Booker |
NT | |
| 4 | 100 | EASTON, CHERITH | FR | Bradenton Christian |
NT | |
| 5 | 60 | SMITH, ZIARRE | FR | Booker |
NT | |
| 6 | 62 | EDWARDS, ISAIALYAH | SO | Booker |
NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 3 | 181 | CLARK, CALISHA | JR | Palmetto |
1:25.00 | |
| 4 | 104 | RAINER, BREN | 7 | Bradenton Christian |
1:21.00 | |
| 5 | 215 | BARTLOMIEJCZUK, CAROLINE | JR | Lakewood Ranch |
1:23.00 | |
| 6 | 410 | KENNEDY, LEAH | FR | Parrish |
1:25.00 |