| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 187 | PANTOJA-ROMERO, JADE | 7 | Our Lady of Lourdes Catholic School (Dunedin) | NT | |
| 2 | 57 | BATICADOS, ELIANNAH | 7 | Guardian Angels Catholic School | NT | |
| 3 | 257 | BRANNON, ELIZABETH | 8 | Westlake Christian School | NT | |
| 4 | 184 | JACQUEMOND, EMILY | 7 | Our Lady of Lourdes Catholic School (Dunedin) | NT | |
| 5 | 32 | MOBLEY, KAYLANI | 7 | Academy Prep Center Of Tampa | NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 70 | ABRAHAM, EMILY | 7 | Guardian Angels Catholic School | 1:28.28 | |
| 3 | 244 | TKACZ, MILLER | 7 | Westlake Christian School | 1:26.89 | |
| 4 | 294 | AHLGREN, MIA | 7 | NorthBay Christian Academy | 1:19.86 | |
| 5 | 8 | ROSENDO, ANALIA | 8 | First Lutheran School | 1:22.67 | |
| 6 | 72 | MIKHAEL, OLIVIA | 7 | Guardian Angels Catholic School | 1:27.23 | |
| 7 | 134 | SCHIPPER, PENELOPE | Espiritu Santo Catholic School | 1:37.22 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 113 | ASTRAB, ALLISON | JR | Espiritu Santo Catholic School | 1:18.71 | |
| 2 | 314 | EICHSTAEDT, KORI | 7 | St. Cecelia Catholic Middle School | 1:14.11 | |
| 3 | 320 | FAY, DAYLIN | 8 | St. Cecelia Catholic Middle School | 1:10.51 | |
| 4 | 94 | TOLLIVER, KENNEDY | 8 | Espiritu Santo Catholic School | 1:09.75 | |
| 5 | 254 | PARKER, CLAIRE | 8 | Westlake Christian School | 1:10.50 | |
| 6 | 31 | EXANTUS, FARRAH | 7 | Academy Prep Center Of Tampa | 1:13.82 | |
| 7 | 304 | REY, ELAINE | 8 | St. Cecelia Catholic Middle School | 1:16.65 | |
| 8 | 24 | WINGFIELD, TYRAH | 7 | Academy Prep Center Of Tampa | 1:19.61 |