| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 67 | SHAKER, MOLLIE | 7 | Guardian Angels Catholic School | 36.57 | |
| 2 | 189 | TABOAS, BELLA | 8 | Our Lady of Lourdes Catholic School (Dunedin) | 35.75 | |
| 3 | 242 | NASTASIAK, EMMA | 7 | Westlake Christian School | NT | |
| 4 | 1 | HAMMOND, CLOEY | 8 | First Lutheran School | 41.05 | |
| 5 | 184 | JACQUEMOND, EMILY | 7 | Our Lady of Lourdes Catholic School (Dunedin) | NT | |
| 6 | 325 | BELL, HANNAH | 7 | St. Cecelia Catholic Middle School | NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 103 | FITCH, LILY | SR | Espiritu Santo Catholic School | 34.29 | |
| 3 | 8 | ROSENDO, ANALIA | 8 | First Lutheran School | 33.62 | |
| 4 | 120 | WATERS, ADDISON | Espiritu Santo Catholic School | 32.60 | ||
| 5 | 170 | YOUNG, MAYA | 8 | Our Lady of Lourdes Catholic School (Dunedin) | 32.88 | |
| 6 | 318 | GREBINGER, SOFIA | 8 | St. Cecelia Catholic Middle School | 34.21 | |
| 7 | 150 | CASTRO-LOPEZ, ELENA | 8 | Espiritu Santo Catholic School | 35.49 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 39 | CANNON, LONDON | 8 | Academy Prep Center Of Tampa | 32.46 | |
| 2 | 206 | CRUME, CAILYN | 7 | Westlake Christian School | 32.21 | |
| 3 | 45 | ZBELA, EMILIA | 7 | Guardian Angels Catholic School | 31.98 | |
| 4 | 298 | DOWNES, VIVIAN | 7 | St. Cecelia Catholic Middle School | 29.75 | |
| 5 | 77 | BLY, SOPHIA | 8 | Guardian Angels Catholic School | 31.85 | |
| 6 | 235 | BURNETT, BROOKE | 8 | Westlake Christian School | 32.09 | |
| 7 | 35 | BALLOON, ALANA | 8 | Academy Prep Center Of Tampa | 32.36 | |
| 8 | 33 | JOYNER, TEYLOR | 8 | Academy Prep Center Of Tampa | 32.59 |