| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 666 | JACOBS, ELLA | 5 | Westlake Christian School | NT | |
| 2 | 633 | STANTON, SAWYER | 6 | Westlake Christian School | NT | |
| 3 | 571 | BELEJCHAK, BROOKE | SO | Espiritu Santo Catholic School | NT | |
| 4 | 474 | CURTIS, SERENITY | 7 | Academy Prep Center Of Tampa | NT | |
| 5 | 683 | WARD, MADDY | 5 | St. Cecelia Catholic Middle School | NT | |
| 6 | 577 | WALKER, LILLIAN | 5 | Our Lady of Lourdes Catholic School (Dunedin) | NT | |
| 7 | 461 | MATHIS, ARRAYA | 7 | Academy Prep Center Of Tampa | NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 584 | OTTE, MARLIE-ROSE | 6 | Our Lady of Lourdes Catholic School (Dunedin) | NT | |
| 2 | 572 | MCCAMMON, GABBY | 5 | Espiritu Santo Catholic School | NT | |
| 3 | 546 | TORRIONI, ISABELLE | SO | Espiritu Santo Catholic School | NT | |
| 4 | 503 | MORENO, SOFIA | 6 | Guardian Angels Catholic School | NT | |
| 5 | 706 | WHITE, MARY AVERY | 6 | St. Cecelia Catholic Middle School | NT | |
| 6 | 466 | CUNNINGHAM, JUSTICE | 7 | Academy Prep Center Of Tampa | NT | |
| 7 | 589 | SACHO, ELISE | 6 | Our Lady of Lourdes Catholic School (Dunedin) | 1:18.52 | |
| 8 | 514 | SCHLAU, GABRIELLA | 6 | Guardian Angels Catholic School | 1:28.36 |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 6 | 645 | IBRAHIM, MICHELLE | 6 | Westlake Christian School | NT | |
| 7 | 519 | ZBELA, LILY | 5 | Guardian Angels Catholic School | NT |