| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Safety Harbor MS | A | NT | |||||
| 2 | Dunedin Highland Middle School | A | Holland | Duffy | Tucker | Boorom | 5:21.28 | 5:21.3 |
| 3 | Mangrove Bay Middle School | A | 5:16.00 | |||||
| 4 | Osceola Middle School - Pinellas | B | 5:07.00 | |||||
| 5 | Clearwater Fundamental Middle School | A | More | Rubin | Barthel | Gehring | 5:11.60 | |
| 6 | Madeira Beach Middle School | A | Mehltretter | Hegberg | Lawless | Krajnik | 5:16.65 | |
| 7 | Largo Middle School | A | Lazarev | Reed | Snedeker | Davis | 5:26.00 | District pool meet |
| 8 | Morgan Fitzgerald Middle School | A | Brown | Slattery | Camacho | Putera | 5:35.00 | Pool 2 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Pinellas Park Middle School | A | Jackson | Hill | Stanton | George | 4:55.00 | Area 2 |
| 2 | Palm Harbor Middle School | A | Napier | Krcmar | Moore | Clamons | 4:51.29 | Pool 1 2026 |
| 3 | Carwise Middle School | A | Adams | Gunter | Anderson | Crane | 4:45.70 | |
| 4 | Tarpon Springs Middle School | A | Camp | McCree | Telon | DeSousa | 5.37 | |
| 5 | Seminole Middle School | A | Corry | Koehler | Nelson | Floresca | 4:45.00 | Pinellas County District |
| 6 | John Hopkins Middle School | A | Williams | Laurence | Ortalan | Leonard | 4:47.70 | |
| 7 | James B Sanderlin K-8 IB World School | B | 4:54.00 | |||||
| 8 | Bay Point Middle Sc | A | 5:04.30 |