| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lake Howell |
A | Patterson | Samuels | Funnye' | Thermezi | 3:31.52 | FHSAA 4A District 3 |
| 3 | DeLand |
A | Roman | Hunter | Mandell | Nicholson | NT | |
| 4 | Hagerty |
A | Schneider | Ramsey | Hatfield | Gandy | 3:38.88 | Hagerty Track and Field Invitational 2026 |
| 5 | University (OC) |
A | Nguyen | McDonough | McDonough | Jeffery | 3:44.26 | Hagerty Track and Field Invitational 2026 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lake Brantley |
A | McCarthy | Collier | Woodson | Ayala | 3:26.93 | FHSAA 4A Region 1 |
| 3 | Lake Mary |
A | Jones | Hartsfield | Cosby | Dixon | 3:25.13 | FHSAA 4A Region 1 |
| 4 | Mainland |
A | Estes | Holmes | Thompson | Berry | 3:21.82 | FHSAA 3A Region 2 |
| 5 | Apopka |
A | Booker | Laster | Pierre | Kemp | 3:24.71 | FHSAA 4A Region 1 |
| 6 | Lyman |
A | Simmons | Lambert Rice | Pabon | Laponte | 3:26.45 | FHSAA 4A District 3 |