| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | University (OC) |
A | Smith | Maleka | Garcia-Ocasio | Orr | 58.40 | SCHS Developmental Meet 1 February 18 2026 |
| 3 | Apopka |
A | Harmon | Aungst | Zheng | Foga | NT | |
| 4 | Circle Christian |
A | McIntire | Dullmeyer | McCoy | Jones | 59.26 | FHSAA 1A Region 2 |
| 5 | DeLand |
A | Hill | Hatton-Gray | Smith | Sanchez | 1:02.11 | SCHS Developmental Meet 1 February 18 2026 |
| 6 | Hagerty |
A | Geter | Leger | Penfold | Smith | 54.55 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lake Howell |
A | Anglin | Jacobus | Jackson | Ibanez | 50.44 | FHSAA 4A District 3 |
| 3 | Olympia |
A | Jones | Howard | Coleman | White | 50.02 | Ocoee Relays |
| 4 | Lake Brantley |
A | Fuller | Parks | Bender | White | 48.69 | FHSAA 4A Region 1 |
| 5 | Mainland |
A | DARIUS | Kroytor | Rodney | Lett | 49.01 | FHSAA 3A Region 2 |
| 6 | Lake Mary |
A | Isaacs | Holley | McDavid | Carter | 50.18 | FHSAA 4A District 2 |