| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 320 | SPAINHOWER, Ava | FR | Wesley Chapel |
NT | |
| 2 | 174 | KURPEJOVIC, Sejla | SO | River Ridge |
1:26.49 | Spring Break Invitational 2025 |
| 3 | 444 | FARMER, Emery | JR | Cypress Creek (Wesley Chapel) |
1:18.66 | |
| 4 | 449 | EJENE, Kassidy | SO | Cypress Creek (Wesley Chapel) |
1:06.85 | |
| 5 | 268 | DENNING, Nandi | SR | Wesley Chapel |
1:08.31 | |
| 6 | 332 | JANKOVIC, Maya | JR | Bishop McLaughlin |
1:20.69 | FHSAA 1A District 9 |
| 7 | 518 | GREEN, Xiomara | FR | Cypress Creek (Wesley Chapel) |
NT |