Event Note:
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St Helens | A | Mendoza, Isabelle | Estep, Sophie | Hora, Gabby | Shriver, Lillianna | 53.24 | |
| 3 | Wilsonville | A | Cerdan, Bethany | Burke, Kristen | Grano, Zoey | Greene, Jillian | 50.49 | |
| 4 | Hillsboro | A | Ellsworth, Maddy | Heierle, Jessica | Kerkering, Emma | Saechao, Promise | 49.66 | |
| 5 | La Salle Prep | A | Holenstein, Talya | Griese, Sophie | Herder, Natalie | Minsker, Sarah | 50.03 | |
| 6 | Sandy | A | Thompson, Abigail | Salveter, Marley | Paul, Natalie | Thompson, Madeline | 51.26 | |
| 7 | Milwaukie | A | Sanchez, Arianna | Swim, Jade | Presley, Taylor | Presley, MaKenna | NT |