| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | WAYLAND UNION | B | Renter | Molina-Gonzalez | Niemczyk | Kooiker | NT | |
| 4 | VALLEYWOOD | A | Lara-Zepeda | Whitfield | DeCenzo | Carnegie | 52.75 | |
| 5 | WAYLAND UNION | A | Wright | Rodriguez | Tomaski | Standish | 1:00.24 | |
| 6 | WAYLAND UNION | C | Gaines | Bailey | Bailey | Knowles | NT |