| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Montabella | A | Davidson | Rood | Willis | Shappee | DQ | |
| 4 | Carson City-Crystal | A | Buskirk | Clark | Doherr-Myers | Satterlee | 4:00.05 | |
| 5 | Vestaburg | A | Schram | Gondek | Walker | Schilling | 4:02.87 | |
| 6 | St. Charles | A | Zelinko | Skiles | Keilitz | Rohde | 4:25.72 |