| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Vestaburg | A | Stratton | Schram | Walker | Schilling | 51.56 | |
| 4 | St. Charles | A | Alvarado | Braley | Hafner | Howell | 45.40 | |
| 5 | Mt. Pleasant Sacred Heart | A | Ambrozaitis | Hoeve | Liu | Rose | DNS | |
| 6 | Carson City-Crystal | A | Bigford | Goodsell | Scofield | Smith | 54.06 |