| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HINDALL, Kayla | Ithaca | NT | |
| 2 | KRASKA, Kalysta | Standish Sterling | 1:01.86 | |
| 3 | SMITH, Jordyn | Ithaca | NT | |
| 4 | GAFFKE, Ella | Midland Bullock Creek | 51.30 | |
| 5 | DAVIS, Alicia | Standish Sterling | 58.88 | |
| 6 | NELSON, Kaylyn | Ithaca | NT | |
| 7 | LOR, Serena | Standish Sterling | 1:14.73 | |
| 8 | FISH, Emilee | Ithaca | NT |