| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | MONTABELLA | A | Allen | Graham | Keeler | Pitts | NT | |
| 3 | COLEMAN MS | A | Rooks | Haggart | Begley | Alberto | NT | |
| 4 | MERRILL | A | Reyes | Monk | Harrison | Chapman | 2:16.57 | |
| 5 | ST CHARLES | A | Fiting | Parson | Sutton | Elrod | 2:16.68 | |
| 6 | MT. PLEASANT SACRED HEART MS | A | VanBuskirk | Maxon | Schafer | Smith | NT |