| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | FULTON MS | A | Johnson | Zelinski | Henry | Patmon | NT | |
| 3 | COLEMAN MS | A | Shea | Jenkins | Bowman | Loudenslager | 55.52 | |
| 4 | ST CHARLES | A | Estrada | Alvarado | Thurston | Lake | 51.60 | |
| 5 | MONTABELLA | A | Robinson | Martin | McKinstry | McCarthy | 52.44 | |
| 6 | MERRILL | A | Delong | Tripp-Campbell | Thompson | Fink | 58.27 |