| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | BRECKENRIDGE | A | Bartlett | Vine | Bartlett | Burt | 4:49.14 | |
| 4 | MERRILL | A | Badour | Wood | Schoch | Frost | 4:15.73 | |
| 5 | MT. PLEASANT SACRED HEART MS | A | Bliss | Dye | Chapman | McQuaid | 4:36.86 | |
| 6 | ST CHARLES | A | Estrada | Thurston | Thurston | Lake | NT |