| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | CARSON CITY-CRYSTAL | A | Jasso | Sorrell | Coon | Coon | 1:07.94 | |
| 2 | BRECKENRIDGE | A | Mayer | Short | Libby | Moulton | 59.00 | |
| 3 | MONTABELLA | A | Lumbert | Fulk | Curtiss | Binge | 55.67 | |
| 4 | ST. CHARLES | A | Estrada | Mose | Zelinko | Hafner | 54.38 | |
| 5 | MERRILL | A | Pappas | Monk | Colpean | Acker | 55.34 | |
| 6 | MT. PLEASANT SACRED HEART | A | Terwilliger | Rose | Predum | Theisen | 57.00 | |
| 7 | FULTON | A | Abrams | Peters | Podolak | Wesenberg | 1:07.14 |