| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | CARSON CITY-CRYSTAL | A | Bigford | Goodsell | Scofield | Smith | 54.06 | |
| 4 | ST. CHARLES | A | Alvarado | Braley | Hafner | Howell | 45.40 | |
| 5 | ST. CHARLES | B | Wicke | Rohde | Wicke | Thompson | 48.50 | |
| 6 | VESTABURG | A | Stratton | Schram | Walker | Schilling | 54.89 |