| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Breckenridge | A | Keller | Gilbert | Waite | Dutton | NT | |
| 4 | Vestaburg | A | Walker | Stratton | Schram | Schilling | 51.56 | |
| 5 | Carson City-Crystal | A | Bigford | Goodsell | Smith | Scofield | 54.06 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fulton | A | Litwiller | Podolak | Diana | McCormick | 49.50 | |
| 3 | Mt. Pleasant Sacred Heart | A | Rose | Middleton | Ambrozaitis | Boykin-Lynch | 47.60 | |
| 4 | St. Charles | A | Howell | Alvarado | Hafner | Braley | 45.40 | |
| 5 | Montabella | A | Arganbright | Kauffman | Rood | Rodriguez | 46.18 | |
| 6 | Coleman | A | Curtis | Lawrence | Martinez | Wright | 47.63 | |
| 7 | St. Charles | B | Wicke | Cameron | Powlus | Wicke | 50.54 |