| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Gladwin | A | Schmidt | Muma | Vannest | Grant | 59.35 | |
| 4 | Midland Bullock Creek | A | Vican | Gaus | LeViere | Bradshaw-Hillabrand | 52.45 | |
| 5 | Ogemaw Heights | A | McDaniel | Hintz | Dent | Starr | 59.00 | |
| 6 | Gladwin | B | Anderson | Sapienza | Brackrog | Lathrop | 1:05.30 |