| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Beaverton | A | Avila | Hubble | Arndt | Murrell | NT | |
| 4 | Midland Bullock Creek | A | Vican | Gaus | LeViere | Bradshaw-Hillabrand | 53.32 | |
| 5 | Farwell | A | Green | Dailey | Yarhouse | Steinert | 57.10 | |
| 6 | Midland Bullock Creek | B | Marolf | Petsch | Skym | Guest | NT |