| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Richardson, Payton | 7 | CALEDONIA (DUNCAN-KRAFT) | NT | |
| 2 | Cook, Harlow | 7 | CALEDONIA (DUNCAN-KRAFT) | 1:07.00 | |
| 3 | Hendges, Fiona | 7 | CALEDONIA (DUNCAN-KRAFT) | 1:07.00 | |
| 4 | Mulder, Ashtyn | 8 | WAYLAND UNION | NT | |
| 5 | Cromer, Ryleigh | 8 | CALEDONIA (DUNCAN-KRAFT) | 1:07.00 | |
| 6 | Vronko, Charlotte | 8 | WAYLAND UNION | NT | |
| 7 | Lakatos, Lexi | 7 | CALEDONIA (DUNCAN-KRAFT) | 1:07.00 |