| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Pinconning | A | Rau | Artes Grau | Gerow | Wittkopp | 1:00.54 | |
| 3 | Bay City Central | A | Campos | Reinhardt | Frederick | Baldwin | 56.69 | |
| 4 | Bay City Western | A | Barbeau | Knochel | Robinson | McGee | 52.43 | |
| 5 | Bay City John Glenn | A | Figley | Ruppert | Carbeno | Alonzo-Mannie | 53.08 | |
| 6 | Essexville Garber | A | Boynton | Clemmons | Kokaly | Dawkins | 57.58 |