| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St Charles | A | Fiting | Dijak | Risch | Sutton | 1:08.31 | |
| 3 | Carson City-Crystal | A | Randolph | Snyder | Bearden | Heleski | 1:02.77 | |
| 4 | Merrill | A | Reyes | Monk | Harrison | Badder | 58.77 | |
| 5 | Coleman MS | A | Alberto | Sharp-Siel | Begley | Haggart | 1:02.72 | |
| 6 | Montabella | A | Graham | Decker | Keeler | Randall | 1:04.90 |