| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | CARSON CITY-CRYSTAL | A | Coon | Jasso | Sorrell | Coon | 1:07.19 | |
| 2 | MONTABELLA | A | Lumbert | Fulk | Curtiss | Binge | 54.97 | |
| 3 | ST. CHARLES | A | Payne | Mose | Zelinko | Hafner | 52.41 | |
| 4 | BRECKENRIDGE | A | Riedel | Mayer | Denwiddie | Clapp | 51.69 | |
| 5 | MT. PLEASANT SACRED HEART | A | Terwilliger | Sokunbi | Rose | Theisen | 52.07 | |
| 6 | MERRILL | A | Monk | Acker | Pappas | Colpean | 54.06 | |
| 7 | FULTON | A | Podolak | Peters | Verwey | Wesenberg | 1:06.07 |