| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | ST. CHARLES | A | Skiles | Wicke | Samyn | Zelinko | 4:12.05 | |
| 2 | CARSON CITY-CRYSTAL | A | Parmenter | Patch | Scofield | Grovesner | 4:00.05 | |
| 3 | MONTABELLA | A | Davidson | Kauffman | Rood | Shappee | 3:50.79 | |
| 4 | MT. PLEASANT SACRED HEART | A | Lynch | Chaffee | Pollatz | Morrison | 3:33.14 | |
| 5 | BRECKENRIDGE | A | Mayle | Mayle | Chiverton | Wing | 3:39.27 | |
| 6 | VESTABURG | A | Caudill | Gondek | Schram | Walker | 3:57.67 | |
| 7 | MERRILL | A | Wiley | Walker | Coty | Verwey | 4:06.50 |