| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | JEFFERSON (MIDLAND) | B | Kramer | Koch | Huls | Newland | NT |
| 4 | MOUNT PLEASANT | A | Rau | Groves | Horan | Enehiroana | 4:25.39 |
| 5 | JEFFERSON (MIDLAND) | A | Eckerle | Netkowski | Reder | Schihl | 4:42.09 |
| 6 | MOUNT PLEASANT | B | Blythe | Justis | Binder | Hausbeck | NT |