| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | ITHACA | A | Gadlen | Litwiller | Wood | Gilbert | 1:04.41 |
| 3 | CRAMER | A | Grifka | Anderson | Hammad | Hugo | 57.70 |
| 4 | SWAN VALLEY | A | Unger | Stockford | Katz | Felsing | 55.07 |
| 5 | ALMA MS | A | Quinlan | Catlin | Bullard | Hall | 55.70 |
| 6 | ALMA MS | B | Peterson | Yens | Cochran | Gauthier | 1:00.00 |