| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | SWAN VALLEY | B | Stajdl | Rapin | Sova | Riley | NT |
| 2 | ALMA MS | B | Wing | Batista | Dancer | Aguilar | NT |
| 3 | CRAMER | A | Wack | Wood | Nadolny | Jaskiewicz | 59.50 |
| 4 | ALMA MS | A | Wager | Harper-Smith | Zombeck | Slater | 49.45 |
| 5 | SWAN VALLEY | A | Owens | Boehler | Stephens | Mendoza | 50.64 |
| 6 | ITHACA | A | Tucker | Groulx | McDonald | Peet | 1:03.27 |
| 7 | ALMA MS | D | Reist | Chaffin | Beeson | Robinson | NT |
| 8 | ALMA MS | C | Bloss | Emery | Warner | Morrow | NT |