| Athlete | Team | Seed | Note | |
|---|---|---|---|---|
| 1 | VASOLD, Haven | Freeland | 50.81 | |
| 2 | HALL, Ella | Freeland | 51.67 | |
| 3 | SEEMANN, Addie | Freeland | 51.80 | |
| 4 | HARTON, Ava | Clare | 52.00 | |
| 5 | WOODS, Savannah | Clare | 54.90 | |
| 6 | NIXON, Miranda | Chesaning | 55.00 | |
| 7 | GILMORE, Siara | Clare | 55.40 | |
| 8 | KRUGER, Alex | Midland | 56.00 | |
| 9 | GAYLORD, Ella | Benzie Central | 56.50 | |
| 10 | O'MALLEY, Brigid | Midland | 57.00 | |
| 11 | ABBOTT, Hannah | Remus Chippewa Hills | 57.00 | |
| 12 | GILBERT, Lauren | Mt. Pleasant | 58.53 | |
| 13 | TORAIN, Semaj | Midland | 59.00 | |
| 14 | HEERLYN, Emma | Hartland | 59.93 | |
| 15 | GIBSON, Lindsey | Remus Chippewa Hills | 1:02.00 | |
| 16 | SHALTIS, Emma | Hartland | 1:02.71 | |
| 17 | LANCZYNSKI, Julia | Chesaning | NT | |
| 18 | LUCE, Krysta | Chesaning | NT | |
| 19 | WISNIEWSKI, Josephine | Hartland | NT |